GenSAS merged annotation GFF3 file to NCBI .sqn file for NCBI genome submission

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4.5 years ago

zack.saud ▴ 50

Hi,

I have annotated a genome using GenSAS, and I have a merged annotation file containing my predicted genes along with the functional blast protein vs protein and Interproscan results.

I am trying to now make a .sqn file containing the annotations from the GFF3 and the genome assembly fasta files, but I see that the annotation is simply giving an accession number for both the blast result and the Interproscan result, is this normal? Will the NCBI website then recognize these accession numbers and convert them to the correct annotation? Also, I have used the NCBI table2asn tool, but after doing it I don't even see the accession numbers.

Has anyone got any hints or tips of an easier way to submit a fully annotated genome to NCBI?

Many thanks in advance

sequencing assembly genome gene • 991 views

ADD COMMENT • link 4.5 years ago by zack.saud ▴ 50

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see GAG

ADD REPLY • link 4.5 years ago by Juke34 8.9k

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Exactly what I was looking for, bless you! Many thanks

ADD REPLY • link 4.5 years ago by zack.saud ▴ 50

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