How To Find Stranded Rna-Seq Experiments Data

Question

[Leszek]

Anyone have an idea how to find data from stranded RNA-Seq experiments in ENA or SRA? I have tried simple keyword search, but with no success:/

[Neilfws]

Why was this question closed?

[Julien Textoris]

No, and I would (also) be interested in knowing the answer !

[Neilfws]

I'll take that as a vote to reopen. Seems like a reasonable bioinformatics question to me.

[Leszek]

Sorry, I closed it as I have found solution quite soon after posting it just by random trials... Details below

[Neilfws]

Good; it's perfectly fine to reply to your own question if you find the answer, then the community benefits. Feel free to select your own answer as the best one too :)

Answer 1

[Leszek]

Search for Strand-specific[All Fields] AND RNA-seq[All Fields] AND "Saccharomyces cerevisiae"[Organism] in SRA and then explore runs one by one. You should find in Library info like:

• strand-specific sequencing of complementary DNA
• dUTP 2nd strand marking

No idea how to search for those in ENA though...

Hope someone will find it useful.