GATK GenomicsDBImport intervals
0
0
Entering edit mode
4.5 years ago
MAPK ★ 2.1k

I am tryiing to run GenomicsDBImport for exome region of chromosome 2 for whole genome gvcf (multiple samples provided as mylist2.list)

my bed file is in this format:

chr2.bed

chr2    41359   41765
chr2    45307   45927
chr2    45729   46454

mylist2.list

1_S    /544562991/1_S.chr2.g.vcf.gz
2_S    /544562991/2_S.chr2.g.vcf.gz

After running this command:

gatk --java-options "-Xms4G -Xmx32G -DGATK_STACKTRACE_ON_USER_EXCEPTION=true" \
 GenomicsDBImport \
--genomicsdb-workspace-path /home/exomes/SNPs/chr2 \
--batch-size 50 \
--intervals /08-JointCalling/SNPs/chr2.bed \
--sample-name-map /home/mylist2.list \
--tmp-dir=/data/tmp \
--reader-threads 5

I am getting this error below. Can someone please help me understand/resolve this error? org.broadinstitute.hellbender.exceptions.GATKException: Cannot call query with different interval, expected:chr2:41360-41765 queried with: chr2:45308-46454

GATK gvcf • 3.8k views
ADD COMMENT
1
Entering edit mode

I remember at one time GATK GenomicsDBImport did not allow multiple intervals. It seems now this problem has been fixed. See: https://gatkforums.broadinstitute.org/gatk/discussion/13447/genomicsdbimport-intervals. Can you check your GATK version?

ADD REPLY
0
Entering edit mode

Yes that was version issue. It worked on GATK 4.1.7.0. Thanks!

ADD REPLY

Login before adding your answer.

Traffic: 2445 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6