Off topic:DESeq2: How to interpret numbers for independent filtering?
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5.0 years ago
nattzy94 ▴ 60

Hi,

I am doing differential expression using DESeq2 and trying to learn more about independent filtering. I used the function metadata(res)$filterThreshold and got the following numbers but don't quite understand them.

> 66.02757% 
> 6.589014

I understand DESeq2 filters out genes of a mean normalised count below the threshold. In this case, does this mean that DESeq2 has filtered out genes with a mean count of 6.589? And based on this counts threshold, 66% of genes were filtered out of the analysis?

If so, is the mean count threshold abnormally low? Or is this quite normal?

Thank you!

R RNA-Seq • 2.3k views
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