I have a list of genes which I am interested among 5 plant genomes. All of these genes are grouped to Gene Families based on gene pairwise comparison score( similarity score). the format is
#geneName chr GeneFamily_ID start end genome_ID
...
I want to do GO enrichment analysis on that to get GO terms but I do not know how to create the input file for the GO analysis. besides some genomes is not in the standard model.
I plan to use the goatool from https://github.com/tanghaibao/goatools
I am totally new to use that so if you could give me any suggestion, I am appreciated
you can use https://biit.cs.ut.ee/gprofiler/ or Cytoscape based CLuego AAP.