Entering edit mode
4.4 years ago
sunnykevin97
▴
990
HI
I had the readlength of 100 bp, how do i calculate the -s parameter for running Kallisto. Suggestions please.
Another tool? Don't you want to solve the issues you have with your current analysis? I answered this ongoing problem here, https://support.bioconductor.org/p/132018/#132020 and suggest you stick with this pipeline and sort out the issues.
The standard deviation is something you have to derive experimentally, by loading the library on a Bioanalyzer.