Virus Multilocus Sequence Analysis(MLSA)

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4.5 years ago

Salsabil • 0

Hi everyone. This is my first time working on a Multilocus sequence Analysis for a virus. Usually, this technique is based on housekeeping genes in bacteria. But for viruses, I don't know which genes I should focus on. Can anyone please give me some advice concerning this issue. Many thanks!

Virus MLSA sequencing • 770 views

ADD COMMENT • link updated 10 weeks ago by Joe 21k • written 4.5 years ago by Salsabil • 0

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You can do whole genome MLST (what you're calling MLSA). You don't have to select just a subset of genes.

Whether you even could would depend how broad a comparison you're trying to draw. If you're comparing a family of viruses for instance, you might be able to focus on things like polymerases, but as the genomes are so streamlined anyway, its unlikely that you'll have many accessory genes anyway.

ADD REPLY • link 4.4 years ago by Joe 21k

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Hello, Joe. Do you have any tutorial for MLST or MLSA using virus?

ADD REPLY • link 10 weeks ago by henry.f.m.magalhaes • 0

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I don't personally, but I'm sure there will be some you can find online, or papers you can follow.

ADD REPLY • link 10 weeks ago by Joe 21k

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