miRNA analysis: ranking
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4.5 years ago
tushar131189 ▴ 20

Hi I have data for deferentially expressed miRNAs for two different cultivars of a crop under two different treatments. (2 cultivars X 2 treatments = 4 sets of data)

-Can I use log2Fold change values for Heat map construction using all the miRNAs detected commonly in all four sets?

-How to rank the expression data to find the best responsive miRNA? The best responsive miRNA should be determined on the basis of activities in all 4 sets, not in single set. There is R based tool named as "RobustRankAggreg" for such thing I guess. But is there any other simple alternative for that?

Thanks.

sequencing RNA-Seq • 593 views
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Hello everyone....

Is there any method available for ranking the miRNAs/genes other than "R". Because "R" based statistics is far from user friendly for newcomer. Please any kind of help/suggestion is appreciated .

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