Enhanced volcano plot - Over-ride colouring scheme with custom key-value pairs for both log2FC and p value

Question

[kousi31]

I am trying to obtain volcano plot for deseq2 results. I tried Enhanced volcano plot package.

It was easy to follow, however I wish to obtain different colors for up and down regulated genes. Custom over-ride example was easy to plot based on log2FC. However it does not take pvalue cutoff into account.

How to obtain different colour for genes with pvalue cutoff of 10e-16 and log2FC? Kindly suggest an answer!

Answer 1

[Kevin Blighe]

Hi, when you are creating the custom colour named vector, you just need to make use of extra operators, like & (AND) or | (OR).

Kevin