I get this error while creating database on BLAST: BLAST Database creation error: mdb_env_open: Input/output error

Question

[oncelmiran6]

I am trying to do blastp for a research project this is my code:

$ makeblastdb -in refseqlassa.fasta -parse_seqids -out lassadbv2 -dbtype prot

After I run this code it gives this error

Building a new DB, current time: 07/20/2020 09:51:49 New DB name: C:\Program Files\NCBI\blast-2.10.0+\bin\lassadbv2 New DB title: refseqlassa.fasta Sequence type: Protein Keep MBits: T Maximum file size: 1000000000B Adding sequences from FASTA; added 1 sequences in 0.0026305 seconds.

No volumes were created.

BLAST Database creation error: mdb_env_open: Input/output error

[husensofteng]

what blast version do you use? have you tried updating it? also, do you have write permission to the directory?

[fishgolden]

I think you are going to create db files under C:\Program Files\NCBI\blast-2.10.0+\bin\ .

The directory is for installed apps and basically you shouldn't touch anything around there (You don't have a permission to create the files).

Call makeblastdb from the directory where you usually use.