Find Orthologs On Biomart

Question

[manishaB]

Hi there,I am new to biological data analysis,so need some idea and suggestions to solve problems.Hoping for a positive response from all. Thanks in advance.

I need to find 1-to-1 orthologous genes between humans and mouse,using Biomart. Where can I exactly get the section for the same? I can find orthologs but not exactly conserved 1-to-1 orthologs.

[Jean-Karim Heriche]

Once you have the relationships between your genes, just remove the ones that are not one-to-one with a bit of scripting.

[GenoMax]

If it does not have to be done using BioMart then you can find that information from MGI here.

Answer 1

[Shalu Jhanwar]

You can get the list of one-to-one orthologous between human and mouse species using Ensembl Biomart as follows:

1. Go to the https://www.ensembl.org/biomart/martview/. Select Mouse.

2. For attributes, select Gene stable ID, Human gene stable ID and Human homology type

3. Download the file as .tsv and filter the rows with homology type as ortholog_one2one.

This will provide the list of the genes with one to one orthology between mouse and human.