how should I filter call.cns results according to cn depth p_ttest probes weight
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4.7 years ago
linouhao ▴ 10

1 the cnvkit doc seems to be much later than the conda version, and there is not a good explatation of how to filter the final rersults depending on cn depth p_ttest probes weight

2 in the doc https://cnvkit.readthedocs.io/en/stable/pipeline.html?highlight=10x#reference, it mentioned "CNVkit will usually call larger CNAs reliably down to about 10x on-target coverage", so what is the recommended depth for tumor and normal samples, and the lowest depth

3 why the batch.py not including the parameter purity, I have read the code, in the line 201 abd 206,can I revise the code directly and add the parameter purity

4 if I have run the batch command, and I want to use the theta result, can I use the cns file for the export theta, I have tried thta, and it gives a pdf containing the tumot and normal percent, is thta the tumor purity, how can I get the value? and I use the theta, but it get more cna than just use the batch, what is the background reason?

5 how can I make my wes result more close to wgs result?

6 for small target panel, is there a limit of panel size for cnvkit?

thanks a lot

cnv • 1.1k views
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