Pathway RNAseq analysis

Question

[Kairos]

Hello,

So I am beginning with RNAseq analysis, and I have found that after a pathway analysis of differential expression data between two groups of samples, I get routes statistically significant where there are as little as 1 o 2 statistically significant genes.

Is possible that the effects of non-significant genes of a pathway, combine their effects, in order to give a statistically significant pathway?

Thanks!

[rpolicastro]

You should edit your post and describe your methodology in more detail.