Entering edit mode
4.3 years ago
pablo
▴
310
Hi ,
I had consensus reads (ccs.bam) from a PacBio sequencing. I used the assembler hifiasm
to create an assembly on these CCS. I got several .gfa
files but I would like to get some statistics on the assembly : % mapped reads , size of the assembly ...
How could I do this?
Thanks
hifiasm doesn't give you an alignment. You used an assembler to create an assembly.
You're right, I edited my post.
Do you have an idea about my question?
You will need to take your original reads and map them back (
minimap2
) to the assembly you generated. Then generate stats. Size of the assembly should be easy to get as a part ofsamtools idxstats
output of alignment data.