Multiple Sequence Alignment for large sequence alignment

Question

[wxboon_98]

Any tool suggested for the multiple sequence alignment of 2000 sequences with the file size of about 60 MB?

[h.mon]

Hello wxboon_98 , and welcome to Biostars. Please take some minutes to read How To Ask Good Questions On Technical And Scientific Forums and then edit your post to provide more details about your problem.

Answer 1

[Mensur Dlakic]

Ordinarily 2000 proteins would not be a major problem, though it seems like you may have fairly long sequences. Here are several choices:

• https://github.com/refresh-bio/FAMSA
• http://www.clustal.org/ (specifically, Clustal Omega)
• https://www.drive5.com/muscle/