Improving PacBio Assembly with Illumina reads
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4.3 years ago
pablo ▴ 310

Hello,

I had to make an assembly with PacBio reads . It is a bacterial genome (~ 4Mb ) .

For some of the samples, I got 1 contig but for some others, I got 2 to 4 contigs while I would like to get only 1 contig .

Could it be a solution to use Illumina reads and an hybrid assembler to polish my PacBio assemblies?

Bests

Assembly pacbio illumina • 1.2k views
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Thanks for the tips.

I'm probably gonna use Pilon to polish my assemblies with Illumina reads, in order to get 1 contig.

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