Entering edit mode
4.2 years ago
harrydolan.dc
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20
What could cause such a difference in count density? If I cull data with a low raw read count and then normalise will this help even out the variation?
Background: I have 9 samples of RNAseq data 3 control and 6 treated. This data however was gained through two runs of sequencing. I noticed big variations between the old run and the new run so checked the raw read density for differences...
(one control and two treated samples were obtained early on, which have been labeled "old" in the graph; Then 6 more samples were obtained two control and 4 treated, which have been labeled "new"
