TCGA: is it possible to use normal samples from another cancer data set in differential expression analysis?
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4.2 years ago
nova_25 • 0

Normal samples for the cancer data I'm working on are not provided (checked the barcodes). So the question is what could I use instead?

normal sample tcga differential expression rna-seq • 885 views
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Short answer, yes. But it depends on your research question, your cancer type and your downstream analysis. Not only tissue sample, if your design let you, you may want to consider cell line also.

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I'm working on eye and adnexa data. I want to find out if the genes of my interest are overexpressed in that data set.

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What's the TCGA project that you're trying to work with?

You may find something useful on cBioPortal. If you do include another cancer dataset, I would try PCA after quantification step to see how the samples from two different studies align with one another.

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It's the eye and adnexa. Thank you for the link.

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