Entering edit mode
12.5 years ago
Kevin
▴
640
Hi! I am trying to do the below but using tped files generated from vcf tools
perform the genome-wide estimatation of IBD sharing:
plink --noweb --bfile nooutliers --genome --out nooutliersIBD
my command was
plink --noweb --tfile chrY.vcf.gz.plinkformat --genome --out chrY.vcf.gz.plinkformat.g
I had this error
Options in effect:
--noweb
--tfile chrY.vcf.gz.plinkformat
--genome
--out chrY.vcf.gz.plinkformat.g
** For gPLINK compatibility, do not use '.' in --out **
Reading pedigree information from [ chrY.vcf.gz.plinkformat.tfam ]
3000 individuals read from [ chrY.vcf.gz.plinkformat.tfam ]
0 individuals with nonmissing phenotypes
Assuming a disease phenotype (1=unaff, 2=aff, 0=miss)
Missing phenotype value is also -9
0 cases, 0 controls and 3000 missing
0 males, 0 females, and 3000 of unspecified sex
Warning, found 3000 individuals with ambiguous sex codes
These individuals will be set to missing ( or use --allow-no-sex )
Writing list of these individuals to [ chrY.vcf.gz.plinkformat.g.nosex ]
162 (of 162) markers to be included from [ chrY.vcf.gz.plinkformat.tped ]
Before frequency and genotyping pruning, there are 162 SNPs
Converting data to SNP-major format
3000 founders and 0 non-founders found
Total genotyping rate in remaining individuals is nan
0 SNPs failed missingness test ( GENO > 1 )
0 SNPs failed frequency test ( MAF < 0 )
Converting data to Individual-major format
After frequency and genotyping pruning, there are 162 SNPs
After filtering, 0 cases, 0 controls and 3000 missing
After filtering, 0 males, 0 females, and 3000 of unspecified sex
**Warning** this analysis typically requires whole-genome level data
to give accurate results
Writing whole genome IBS/IBD information to [ chrY.vcf.gz.plinkformat.g.genome ]
Filtering output to include pairs with ( 0 <= PI-HAT <= 1 )
IBD(g) calculation: 0 of 6242811
ERROR: No nonmissing markers for individuals 0122 0122 - 0182 0182
As warning suggests
162 SNPs
is not whole-genome level data. And error, I guess, is because0122 0122 - 0182 0182
individuals has noCalls for all SNPs?