Trinity.fasta file missing "does not exist in the out directory:

Question

[bilal.sarwar]

Hello i ran the Trinity for denovo assembly on paired-end RNA-Seq data. it completed successfully after 52h of processing. but the trinity.fasta file is missing in the out folder directory. here is the code that i used

/home/bilal/RNA_Seq_Project/trinityrnaseq-2.2.0/Trinity --seqType fq \
--left /home/bilal/RNA_Seq_Project/left.fastq \
--right /home/bilal/RNA_Seq_Project/right.fastq \
--SS_lib_type RF \
--max_memory 58G \
--CPU 10 \
--output /home/bilal/RNA_Seq_Project/trinity_AssebleSeq

what i missed, any suggestion ..........

Regards

[st.ph.n]

Did you pipe the output to a log file? Did you get an error? I've had no assembly file with the new version of Trinity after running for long hours. I would restart Trinity, keeping all files you have now so it knows where it left off, and you should get your assembly file.

[Medhat]

do you have enough space in your disc?

[eozcan]

did you find a solution for your problem, can you share?

[Ram]

Please do not use answers to ask for updates. Use comments instead.