IPA (Improved Phased Assembly) output questions
1
0
Entering edit mode
4.2 years ago
slin023 • 0

Hello, I just finished IPA assembly for my genome assembly (https://github.com/PacificBiosciences/pbipa), but I received two output files. The tutorial didn't mention anything, I checked two fasta files, one is

  1. final.a_ctg.fasta, it has id named " >hap_ctg.000084F_1 HAPLOTIG", "hap", I assume is haploid?

The other is 2.final.p_ctg.fasta, it has id named ">ctg.000071F_1"

Can someone explain the difference between two files, and what the letters and numbers mean, please?

(ex: hap_ctg.000084F_1, what is ctg? what is 00084? what is F_1?, for two output files, what do "a" and "p" represent in fasta name?)

Assembly genome PacBio • 1.7k views
ADD COMMENT
0
Entering edit mode
4.2 years ago
h.mon 35k

Some of this information can be found at an old Falcon documentation page:

The final output of this step is a fasta file of all of the primary contigs, p_ctg.fa as well as an associated contig fasta file, a_ctg.fa that consists of all of the structural variants from the primary contig assembly.

A cursory look at the wiki revealed no clues, I think you could open an issue at the IPA github page asking for better documentation - just be sure to search extensively before doing so.

ADD COMMENT

Login before adding your answer.

Traffic: 2633 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6