CIBERSORT input file format

Question

[Sara]

I have RNAseq data and their count table. now I would like to use CIBERSORT https://cibersort.stanford.edu/ but it is not clear what the format of its input is. do you guys know what the CIBERSORT input file format is?

Answer 1

[ShahiRB]

It is clearly mentioned here https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895181/ . But the authors recommend CIBERSORTx, the next generation version of CIBERSORT, with support for single-cell RNA-seq and cell type-specific gene expression purification.

[Shicheng Guo]

CIBERSORTx used no-negative matrix decomposition or not?