Can anybody suggest the bioinformatics approach for studing gene regulation by miRNA? or any case study...
Can anybody suggest the bioinformatics approach for studing gene regulation by miRNA? or any case study...
miRNAs control gene expression by complementary base pairing to mRNAs. Read about RNA induced silencing complex (RISC) for more details.
Hi Chirag, I think that would be a good question for a molecular biology forum. From what I understand in a DICER deficient cell, the can be no miRNA loaded RISC complex because pre-miRNA transcripts cannot be cleaved, however artificial siRNA would still integrate into RISC and could induce transcriptional knock-down.
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be more specific ? have you sequencing data ?
simply read the others questions tagged mirna will show you a lot of different approaches. As you can imagine there is not only a single 'the bioinformatics' approach.