Mirna
1
0
Entering edit mode
12.4 years ago
Mandar ▴ 180

Can anybody suggest the bioinformatics approach for studing gene regulation by miRNA? or any case study...

mirna • 2.2k views
ADD COMMENT
0
Entering edit mode

be more specific ? have you sequencing data ?

ADD REPLY
0
Entering edit mode

simply read the others questions tagged mirna will show you a lot of different approaches. As you can imagine there is not only a single 'the bioinformatics' approach.

ADD REPLY
0
Entering edit mode
12.3 years ago
Pappu ★ 2.1k

miRNAs control gene expression by complementary base pairing to mRNAs. Read about RNA induced silencing complex (RISC) for more details.

ADD COMMENT
0
Entering edit mode

In the DICER mutant cell, there will be no mature miRNA and hence not loaded with AGO2 in RISC complex. In such scenario, when no siRNA sequence is loaded, can miRISC can be recruited to 3'UTR or not ?

ADD REPLY
0
Entering edit mode

Hi Chirag, I think that would be a good question for a molecular biology forum. From what I understand in a DICER deficient cell, the can be no miRNA loaded RISC complex because pre-miRNA transcripts cannot be cleaved, however artificial siRNA would still integrate into RISC and could induce transcriptional knock-down.

ADD REPLY

Login before adding your answer.

Traffic: 932 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6