real time pcr primer design

Question

[sh]

Hi every one i have one question it might be simple!!! i was designed real time pcr primer for my gene by using Alleleid software then i performed primerblast. the result showed there are isoforms for this gene.now i don't what am i do, Could anyone help me?

[seta]

You can design the primers from the conserved region of different isoforms if you have no idea which isoform is expressed in your experiment.

[sh]

Sorry i have a silly question; how can i design the primers from the conserved region of isoforms? thanks alot

[ATpoint]

Are you interested in the total gene expression or in an individual isoform?

[sh]

total gene expression

[ATpoint]

Then simply load the NCBI gene name into PrimerBLAST and use what it outputs.

[sh]

thanks for your help