Multi-group DE scRNA-seq comps without replicates

Question

[LacquerHed]

Wondering if its possible to do multi-group DE comparisons of 10X/Seurat data without replicates, using perhaps just the per-cell counts alone? To compare DE genes across clusters from lets say 2 genotypes and 2 treatments.Thanks.

Answer 1

[khorms]

Yes there are definitely methods for DE comparisons implemented in the commonly used single cell packages; for example, see this discussion (link) for scanpy

[LacquerHed]

Do you know of any good tutorials for doing this in Seurat? Thanks.