In the paper from Hwang et al. (Science 367, 1255–1260 (2020) ) there is a mention about normalizing cytoplasmic mRNA abundance to that of mitochondrial RNA
exact citation is this
We validated the global increase by normalizing cytoplasmic mRNA abundance to that of mitochondrial genome–encoded mRNAs (mtRNAs) and spike- in RNAs (materials and methods) (17, 18).
Unfortunately there is no more elaboration in the method part and nothing that i could find in the two references (17,18). I would appreciate it if someone can explain to me how this kind of normalization is done.
Would it be as simple as taking the averaged sum of the mtRNAs as a denominator in the calculation, while each of the cytoplasmic genes as a numerator? would this division give me the normalized abundance of cytoplasmic gene expression normalized against the mitochondrial gene expression?
thanks Assa
