Dear community,
I have a very little quick question: I have directional paired-end RNAseq data which I want to put in the UCSC genome browser in a form which the same track displays as positive values forward reads and negative values reverse reads. Do you know a way to do it?
Below I paste an image found on google to show an example of what I want to do:
Thanks for your help!!!!!
you assumed that forward and reverse reads do not overlap, which may not be true.
Good point — will try to come back to this answer with an update at some point.