GO enrichment based on own DEG and GO annotation
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4.1 years ago
biosjm • 0

Hello, In my RNAseq project I have generated DEG results by Deseq2 and I have a GO annotation dataframe (gene id with GO numbers), so it`s not a model organism. I was starting to analyze GO enrichment with TopGo, which worked well and I have a lot of enriched GO terms. But to get a better overview, I would like to plot them, preferred as barplot of up-regulated and down-regulated genes (probably separate for Biological Process, Molecular Function, Cellular Component) to get a better overview. Also it would make sense to assign them to categories, such as reproduction, metabolic....

I know there a lot of packages for Rstudio available but mainly usable for model organisms and they use public online annotation databases.

Can you recommend any package, script, vignette for using a custom annotation?

Thanks a lot!

go enrichment DEG RNA-Seq • 790 views
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