Entering edit mode
4.0 years ago
biohacker_tobe
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80
I have done the aligning of my protein sequences and of short reads against a protein reference database using diamond. However I would like to annotate my alignments but I would prefer to do this on bash. I found this https://resources.qiagenbioinformatics.com/manuals/clcmgm/current/index.php?manual=Annotate_with_DIAMOND.html to be useful but this is a seperate software.
Question: Is it is possible to annotate this alignment created from Diamond within bash?
Hi,
First of all, what do you mean with "annotate". If you are simply talking about adding names/descriptions to your alignments, you can easily do it with
grep
,awk
,sed
, etc, depending on what format of your outputs (e.g. if you got any tabular file of your input/query IDs versus your hits/subject IDs). Also, the proteins database is a well documented/curated one (like nr, refseq, swissprot) or is it a cusomized set of yours?Cheers