Entering edit mode
4.0 years ago
jocelyn.petitto
▴
20
I posted about this issue in the git for kallisto ( https://github.com/pachterlab/kallisto/issues/286 ) but I am trying to determine what to do in the meantime. Why would I be getting an output with a *.bin instead of a *.bam ?
Ultimately, I need the read counts re: what fragment is being ascribed to what via the kallisto algorithm as I have non-canonical mitochondrial ncRNA that have sequence similarity with another gene and must figure out a statistical method for properly ascribing reads to transcripts.
Is that an unfortunate spelling error on programmer's part? Have you tried to rename
.bin
to.bam
and checking withsamtools
to see if it is indeed a BAM file?