Generating Taxonomy and Sequence References for Microbial Functional Genes Using Qiime2.

Question

[jazebe_1461]

Hi all, As you know, analyzing 16S (housekeeping gene) sequence by Qiime2 is required to provide taxonomy and sequence references. We can find them in the SILVA database. I essentially need taxonomy and sequence references for analyzing my functional genes data to go through the downstream processes. To generate them, what I was told, one should apply from NCBI website tools, such as FTP, e-utility and e-fetch, which my knowledge in this area is sadly rudimentary. I need your assistance to give me an in-detailed protocol to generate them. I am looking forward to hearing from you. Thanks a lot.

[h.mon]

Probably this paper will help a lot:

Bioconductor Workflow for Microbiome Data Analysis: from raw reads to community analyses