Sequences(Without Ref Genome) For Pathway Analysis?

Question

[zhaoqi699]

hi! here is my problem: i have some sequence assembled by trinity ,with raw data and downstream Trinity scripts ,a list of Differencial Expression seqs were collected. i wanna do GO and Kegg analysis with those sequences and i have no idea on how to do it cause there is no reference genome .so can anyone give me some advice or recommand some software to do this? thanks!

Answer 1

[Nicolas Rosewick]

try blast2go http://www.blast2go.com/b2ghome

Answer 2

[zhaoqi699]

thanks for your reply,i 've used this software,but but it is too slow to annoted my sequence about 200,000(as background to calculate the Erichment as well as P-value),