Not able to find barcode file for Spatial Transcriptomic data

Question

[Mahesh Rao]

I am a student who's taking a course in computational genomics and I wanted to try this tutorial in Seurat for which I have to create a Seurat object. According to the documentation for creating the Seurat object, along with the count matrix, a barcode file containing the spot barcode and x and y coordinates is required. I am referring to this dataset, in which the raw count matrix is provided but I'm not able to find a barcode file for the given raw count matrix.

Am I missing something obvious or is the barcode file not there for this dataset ? If it isn't there, is there a way to derive the barcode file from the dataset ?

Answer 1

[markus.riester]

Seurat only supports Visium, not the older ST assay. I have a wrapper in https://github.com/lima1/sttkit that generates a Seurat object compatible with all their Spatial* functions.