The Niehrs laboratory studies regulation of DNA methylation, which plays important roles in development & disease. We have recently demonstrated a role for long non-coding RNAs and R-loop DNA:RNA hybrids in DNA methylation (Arab et al. 2014; Arab et al. 2019). In an ERC-funded project, we now aim to elucidate the mechanisms of DNA demethylation as well as the role played by long non-coding RNAs and R-loops in its regulation. See also https://www.imb.de/research/niehrs/research
The Project
Candidates will make use of mouse embryonic stem cells (ESCs) to interrogate genome-wide the role of ncRNAs and R-loops in targeting GADD45a and the DNA demethylation machinery in the genome. We will use CRISPR/CAS9 mediated ESC mutants, as well as ChIP-seq, RNA-seq, bisulfite-seq to carry out multi- dimensional data analysis to address:
Where in the genome does demethylation occur? What are the molecular determinants in ncRNA & R-loops involved in targeting? What are the cofactors involved? The project will involve strong interaction with a Computational Biologist
Required Qualifications
- PhD & publications in a relevant field
- Sound experience in molecular biology
- Familiarity with Omics techniques or –analysis, or with ncRNAs or R-loops is advantageous
- Excellent communication skills and good team spirit with the ability to solve problems independently
- Fluent in English (spoken and written)
How to apply
Please see details on the project, information on how to apply, and an online application form at http://www.imb.de/postdocs. IMB is an equal opportunity employer.
The deadline for applications is 28 January 2021. Interviews will take place online. Interview dates will be announced once available.
Application webpage: http://www.imb.de/postdocs
