Off topic:Differential expression between just 2 samples: a normal and a tumour sample from the same patient

Question

[jeni]

Hi!

I am trying to perform some differencial expression analysis just to know which genes present different expression levels between a normal and a tumoral samples from the same patient. I have aligned and obtained gene counts with STAR and now I am trying to use DESeq2 to find differences between both samples. However, as I only have 1 sample for each group (tumour vs normal), DESeq2 is not working.

Do you know how can I achieve this goal?

Thanks!