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4.1 years ago
whb
▴
60
Hi I am a new user of parallel. I am trying to use it instead of loops to do fastq file trimming using Agilent Agent trimmer. When I run it one by one without using parallel, output is one R1 and one R3 file for each sample as expected. But using the code below produced 4 R1 and 4 R3 files for each sample. Could someone tell me what I have done wrong please?
Input:
cat metatry.txt | parallel java -Xmx5G -jar $Trimmer -fq1 {}R1_001.fastq.gz -fq2 {}R3_001.fastq.gz -xt -out_loc /Trimmed/
Output:
trim -fq1 153_D1_S11_R1_001.fastq.gz -fq2 153_D1_S11_R3_001.fastq.gz -xt -out_loc /Trimmed/
Trimmed file created:
/Trimmed/153_D1_S11_R1_001.1607137111246_Cut_0.fastq.gz
/Trimmed/153_D1_S11_R3_001.1607137111246_Cut_0.fastq.gz
For Research Use Only. Not for use in diagnostic procedures
(c) Agilent Technologies, Inc. 2016-2019. Version: 2.0.2. Please report problems to informatics_support@agilent.com
cd /Trimmed/
153_D1_S9_R1_001.1607136132795_Cut_0.fastq.gz
153_D1_S9_R1_001.1607136580643_Cut_0.fastq.gz
153_D1_S9_R1_001.1607136902485_Cut_0.fastq.gz
153_D1_S9_R1_001.1607137111246_Cut_0.fastq.gz
153_D1_S9_R3_001.1607136132795_Cut_0.fastq.gz
153_D1_S9_R3_001.1607136580643_Cut_0.fastq.gz
153_D1_S9_R3_001.1607136902485_Cut_0.fastq.gz
153_D1_S9_R3_001.1607137111246_Cut_0.fastq.gz
The output of
head metatry.txtwould be useful here.Show us the output of
parallel --dry-run ...