what is the best way to find ortholog protein in other bacterial strains ?
what is the best way to find ortholog protein in other bacterial strains ?
The best way to find orthologs is to read some posts that have already discussed a similar problem and do some reading.
A few of the posts on Biostars that discuss the same are:
What is the best method to find orthologous genes of a species?
Simplest way is to do a bi directional blast !!
My group prepared a very nice tool based on KEGG ( see https://dariusz-izak-doktorat.github.io/ )
Sorry about it. It should be https://dariusz-izak-doktorat.github.io/
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you can use orthofinder, which is easy to run and produces many useful comparative genomics results as well as phylogenetic trees inculuding species tree and ortholog trees.
here is the link