Removing warning FASTQC

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3.8 years ago

ipb727258 • 0

I wish to know how can I fix warning per tile sequence quality, per base sequent content and per sequence duplication

or Do I have ignore these errors, does not affect downstram analysis Thank you

next-gen • 840 views

ADD COMMENT • link 3.8 years ago by ipb727258 • 0

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Is this DNA or RNA? It matters.

ADD REPLY • link 3.8 years ago by swbarnes2 14k

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It is bacterial genom/DNA

ADD REPLY • link 3.8 years ago by ipb727258 • 0

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ADD REPLY • link 3.8 years ago by Ram 44k

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While not all of these are likely to apply in your case take a look at https://sequencing.qcfail.com/articles/libraries-can-contain-technical-duplication/ and some other informative blog posts about interpreting FastQC results but authors of FastQC here.

ADD REPLY • link 3.8 years ago by GenoMax 147k

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