What are those black lines in my heatmap?

Question

[grx0326]

I have been using deeptools plotHeatmap to generate heatmap around TSS regions from my chip-seq data.
However there are black lines in my heatmap and I don`t know what those lines stand for.
Here is my code:
plotHeatmap -m [prefix]matrix.gz -o [prefix]normalized.png --boxAroundHeatmaps no --colorMap bwr --heatmapHeight 15 --heatmapWidth 3 --whatToShow 'heatmap and colorbar' & And here is what I got:

Any ideas are appreciated

Answer 1

[Devon Ryan]

They're missing data. You can change the color if you like.

[grx0326]

you mean change the black line into other color？I am not sure how to achieve that

[Devon Ryan]

It's the --missingDataColor option, see the online help page for more information: https://deeptools.readthedocs.io/en/develop/content/tools/plotHeatmap.html