Pairwise distance matrix using biopython

Question

[Elephantdumbo]

Hello, I am wondering what is the most efficient way to calculate a distance matrix for a set of 400 sequences each is ~120 bp in length. I would prefer not use an outside tool but do it all in python

Thank you so much

[Joe]

If you already have an alignment, you can do this with the Phylo tools in biopython: https://biopython.readthedocs.io/en/latest/api/Bio.Phylo.TreeConstruction.html