Question

Unable To Go Through Biopython'S Motif Tutorial - Ioerror

1

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12.5 years ago

Olivier ▴ 440

Hello

I'm trying the Sequence motif analysis tutorial in the Biopython cookbook and it's not working. Can someone help? Here's how it is:

from Bio import Motif
arnt = Motif.read(open("Arnt.sites"), "jaspar-sites")
Traceback (most recent call last):
  File "<pyshell#1>", line 1, in <module>
    arnt = Motif.read(open("Arnt.sites"), "jaspar-sites")
IOError: [Errno 2] No such file or directory: 'Arnt.sites'

biopython motif • 3.1k views

ADD COMMENT • link updated 12.5 years ago by Leonor Palmeira 3.9k • written 12.5 years ago by Olivier ▴ 440

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as it says: the file 'Arnt.sites' doesn't exist in your current working directory.

ADD REPLY • link 12.5 years ago by Pierre Lindenbaum 164k

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You're right Pierre. But its location is not mentioned in the tutorial. Do you have an idea what "Arnt.sites" and "jaspar-sites" refer to? It's about JASPAR-formatted motifs but I don't know how to carry on.

ADD REPLY • link 12.5 years ago by Olivier ▴ 440

score 5 · Answer 1 · 2012-07-13

5

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12.5 years ago

Leonor Palmeira 3.9k

These Arnt.sites and SRF.pfm from the BioPython manual are from the JASPAR database. Somewhere in here: http://jaspar.genereg.net/html/DOWNLOAD/

These are just example files to fill the manual, you should make your own in case you're doing your own analysis.

ADD COMMENT • link 12.5 years ago by Leonor Palmeira 3.9k

score 1 · Answer 2 · 2012-07-13

1

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12.5 years ago

Istvan Albert 102k

add the Arnt.sites file to your work directory

ADD COMMENT • link 12.5 years ago by Istvan Albert 102k

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Could you please tell me where I can get this file? I can't really find it on the JASPAR database... unless it's something like this 'MA0001.1.sites'? Thanks