How To Convert Bigwig To Bam?

Question

[ashis.csedu]

I am working with some bigwig data that were generated from bedGraph data from an RNA-seq experiment. Since the bigwig files I have list coverage scores with 1bp span. These bigwig/bedGraphs were generated from BAM files that I don't have, but can I re-create those BAM files from BigWigs? Thanks.

Answer 1

[Jorge Amigo]

unfortunately not. BAM files are alignments, reads that came out from a sequencing experiment and that have been mapped to a reference sequence. bigwig and bedgraph files are just informative for coverage, number of reads for each particular site of that reference sequence, like summaries of what the original BAM file had. you can't generate the original BAM file from the coverage file: it would be as "I have this SNP allele frequency for all populations around the world, can I get all the genotypes of all the individual samples from it?". it's just a conceptual mistake.

[ashis.csedu]

Thanks for clarifying the mis-understanding. It helps.