fastqc report and quality check

Question

[aranyak111]

I am conducting an RNA seq analysis pipeline for which I have to conduct fastqc report check. I am curious to know what is the exact significance of the parameter of the term per tile sequence quality. If this parameter seems problematic and other quality checks are okay can we still proceed with the fastq file?

[GenoMax]

Can you post an image: How to add images to a Biostars post

[aranyak111]

enter link description here

Let me know if you can access the link.

[GenoMax]

You can't directly link files from your local desktop. Please upload them to imgbb dot com following directions in post above.

[aranyak111]

Please let me know if you can see the uploaded image and subsequently give your valuable opinion.

[Medhat]

I changed that to a comment, because it is not an answer, and we can not see the image you uploaded, refer to GenoMax comment.

[desouzareis.r]

You can find useful information here

Answer 1

[JC]

It depends how bad it looks like, a bubble or areas with errors are ignored in the base calling, details: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/3%20Analysis%20Modules/12%20Per%20Tile%20Sequence%20Quality.html

[aranyak111]

![enter image description here][1]

https://ibb.co/Lvs51mF

[GenoMax]

Not the plot you wanted to show us.