Blast Azure VM

Question

[anasofiamoreira94]

I'm using azure vm of blast, which as blast software version: 2.2.25 And I installed the most recent version of blast database.  However, when I do blasdbcmd -db nt -info I get this error: BLAST Database error: Error: Not a valid version 4 database. Can I update blastn version in this vm, or should I do this in a different VM? Thanks for the help

Answer 1

[GenoMax]

I don't know what you mean by azure vm of blast. If this is a regular VM otherwise, you should be able to use conda to install latest blast+ or download pre-compiled binaries if conda is not an option.

The reason things don't work is because current blast databases are v.5 and require blast+ of recent vintage. If you must use v4 databases (which are required for blast v.2.2.25) you can find an year old version on NCBI blast db site here. Be aware that NCBI does not update these old format db.

[anasofiamoreira94]

Hi @GenoMax, thanks for the answer. I don't want to use v4 databases, because some species aren't correctly annotated. So, I will try to update the blast version in the vm.