Gene expression values each samples (replicates) using `AverageExpression` function
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3.8 years ago
singcell • 0

I have performed single-cell experiment containing 3 Ctrl and 3 treatment (T) samples and I used Seurat integration vignette for comparing Ctrl and T conditions. I use AverageExpression function to get the expression values of genes in Ctrl and T samples in various clusters. However AverageExpression displays the combined expression values of all Ctrl and T samples for each cluster.

I want to determine the individual expression values of genes separately for each samples of Ctrl and T (from integrated Seurat object). I am wondering how can I get individual expression values

Something like this:

Cluster 1

--------------Ctrl1--------- Ctrl2---------Ctrl3-------T1----------T2----------T3

GAPDH----10------------15 ----------13--------- 5-------------17------------9

ACTB------10------------15 ----------13--------- 5-------------17------------9

Cluster 2

-------------Ctrl1--------- Ctrl2---------Ctrl3-------T1----------T2----------T3

GAPDH----10------------15 ----------13--------- 5-------------17------------9

ACTB------10------------15 ----------13--------- 5-------------17------------9

Is it possible to get this info from integrated data?

Thank you

SEURAT SINGLE-CELL averageexpression • 726 views
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