How RNAseq input file for ssGSAE should be normalized?

Question

[Raheleh]

Hi all,

I have RNAseq of 12 mouse models (4 genotypes) that are vst normalized using Deseq2. I want to do ssGSEA analysis to see the enrichment of hallmark geneset for each sample. Should they be normalized for gene length as well?

Many thanks for your help!