Entering edit mode
6.6 years ago
Peter Chung
▴
210
I am new in sequencing analysis and would like to do haplotype blocks from the result of WGS. But I have some questions on the HAPCUT2.
I generated a vcf from gatk and then I phased it using gatk ReadBackedPhasing into phased vcf.
Commands below are from HAPCUT2:
./build/extractHAIRS [options] --bam reads.sorted.bam --VCF variants.VCF --out fragment_file
./build/HAPCUT2 --fragments fragment_file --vcf variantcalls.vcf --output haplotype_output_file
I saw there are two vcf files in different names, so which one should I input my phased vcf and what is another vcf file ? btw, is it better to put a phased vcf in HAPCUT2 ?
Thank you.
Please use the formatting bar (especially the
code
option) to present your post better. I've done it for you this time.hello, did you figure it out which vcf file should be used. I only have one vcf file output from the WGS analysis