read10X ERROR Seurat

Question

[GiuliaAC]

Hi there

I'm analysing single cell RNA seq data with seurat, but I got the following error message

I have to say that is NOT the first time I analysed single cell data, but it is the first time I got this error

sampleHD01 <- Read10X(data.dir = pathofthefirstsample)
Error in type.convert.default(data[[i]], as.is = as.is[i], dec = dec,  :
  invalid multibyte string at '<dc>ޑ<e5><f2><fc><3f><e0><ba><df><a9><f4><74><03><58><57><68><be><2b><a2><47><b5><15><ff><76><16><38><63><d3><ad><c5><62><b9><0b><9a><af><c6><8d><12><47><1d><d9><25><d9><6e><2d><25><3b><59><6e><45><97><c4><32><96><ea><d2><c3><10><90><c8><37><bc><43><0f><0b><a7><7e><35><e3><4e><4d><d9><e6><98><6c><65><d4><76><1f><4d><7f><a4><26>'
Inoltre: Warning messages:
1: In read.table(file = file, header = header, sep = sep, quote = quote,  :
  line 1 appears to contain embedded nulls
2: In read.table(file = file, header = header, sep = sep, quote = quote,  :
  line 4 appears to contain embedded nulls
3: In scan(file = file, what = what, sep = sep, quote = quote, dec = dec,  :
  embedded nul(s) found in input

Thank to all of you for your help

Giulia

[rpolicastro]

Do you get this error with all of your samples, or just that one?

[GiuliaAC]

I got this error message with only that sample With the other sample, the line works