How to identify the reads from diffient seq-data is from the same gene?

Question

[972050141]

First I THINK this reads is from the gene Second I can’t identify if the reads are from the gene? IF we have the same standard to identify where the reads from?(if only one base coincidence?)

[972050141]

picture https://i.ibb.co/1KL4hft/1.png

[rpolicastro]

It's possible for the reads to overhang from the annotated gene. The TSS or TES could be incompletely annotated, there could be a novel splice isoform, or you're getting a larger 5' or 3' UTR. I think the best evidence would be having numerous reads covering that region.