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3.8 years ago
daewowo
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80
I have been trying to find a method to extract a consensus from de-novo assembled contigs against a reference genome. This is how I currently do this:
De-novo assemble the dataset. Identify sequence of interest in it. Run bowtie2/bwa mem to align the contigs against a reference genome of interest. Use igv to extract consensus.
Is there any other tool that can generate a consensus sequence from contigs and a reference genome?
Hi,
Have you looked at this post? It may help.